The Effect of High-intensity Interval Training and L-carnitine on the Expression of Some Pro-inflammatory Genes in the Liver and Cardiac Tissues of Rats

Q: What are the key findings of this study?

High-intensity interval training plus L-carnitine significantly reduced hepatic IL-6 and Cox-2 and cardiac IL-1β expression compared with untreated controls in rats (all p<0.001).

Q: What is the clinical evidence from this study?

Population: Exercise-induced and baseline tissue inflammation in healthy male Wistar rats (n=32). Intervention: High-intensity interval training (HIIT) combined with L-carnitine (LCAR) vs. Untreated control, L-carnitine alone (200 mg/kg/day), or HIIT alone. Primary outcome: Relative expression of pro-inflammatory genes (IL-1β, IL-6, TNF-α, and Cox-2) in liver and cardiac tissues (p=<0.001).

Q: What does this research mean for the field?

Combining high-intensity interval training with L-carnitine supplementation significantly attenuates pro-inflammatory gene expression in rat liver and cardiac tissues. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

Q: What question did this study set out to answer?

To determine the effects of high-intensity interval training (HIIT) and L-carnitine (LCAR) on the expression of pro-inflammatory genes in the liver and cardiac tissues of rats.

Beydolah Shahouzehi; Hamidreza Nasri; Soheil Aminizadeh; Yaser Masoumi‐Ardakani

doi:10.22062/jkmu.2021.91564

January 1, 2021Majallah-i dānishgāh-i ̒ulum-i pizishkī-i Kirmān.Open Access

The Effect of High-intensity Interval Training and L-carnitine on the Expression of Some Pro-inflammatory Genes in the Liver and Cardiac Tissues of Rats

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Key result

HIIT plus L-carnitine significantly reduces hepatic and cardiac inflammatory markers in rats.

P<0.001
n=32

Why the study?

The effects of high-intensity interval training and L-Carnitine on the expression of genes involved in inflammation and inflammatory pathways in rat liver and heart tissues needed to be determined.

Key Points

To determine the effects of high-intensity interval training (HIIT) and L-carnitine (LCAR) on the expression of pro-inflammatory genes in the liver and cardiac tissues of rats.
Thirty-two male Wistar rats were randomly divided into four groups (n = 8 per group): untreated control, LCAR (200 mg/kg daily), HIIT, and HIIT + LCAR.
Gene expression of pro-inflammatory markers (IL-1β, IL-6, TNF-α, and Cox 2) relative to 18s rRNA was quantified in liver and cardiac tissues using real-time PCR.
In liver tissue, HIIT + LCAR significantly reduced IL-1β expression compared to HIIT alone (p = 0.038), decreased IL-6 compared to control (p < 0.001), LCAR (p < 0.001), and HIIT (p = 0.002), and decreased Cox 2 compared to control (p < 0.001) and LCAR (p = 0.007).
In cardiac tissue, HIIT + LCAR significantly reduced IL-1β expression compared to control (p < 0.001), LCAR (p = 0.034), and HIIT (p = 0.041), and reduced TNF-α and Cox 2 compared to other groups (p < 0.05).
In cardiac tissue, HIIT + LCAR significantly increased IL-6 expression compared to the control, LCAR, and HIIT groups (p < 0.001).

PICO

P

Population

32 healthy 8-week-old male Wistar rats randomized into four groups and followed for 4 weeks of intervention.

I

Intervention / Comparator

High-intensity interval training (HIIT) combined with L-carnitine (LCAR) vs Untreated control, L-carnitine alone (200 mg/kg/day), or HIIT alone (200 mg/kg/day intraperitoneal L-carnitine plus 30 min/day HIIT (10 repetitions of 1 min with 2 min active rest, 5 days/week))

O

Primary Outcome

Relative expression of pro-inflammatory genes (IL-1β, IL-6, TNF-α, and Cox-2) in liver and cardiac tissues, p=<0.001

Main Result

p-value: p=<0.001

Limitations

Preclinical animal model (male Wistar rats) limiting direct clinical generalizability to humans
Only one type of exercise training (HIIT) was evaluated without direct comparison to other training modalities such as classical endurance exercise
Small sample size (n=8 per group)

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Implication

Demonstrates that combining high-intensity interval training with L-carnitine reduces pro-inflammatory gene expression in rat liver and cardiac tissues, indicating potential anti-inflammatory...

Abstract

Background: Inflammation is characterized by interactions between cytokines and inflammatory pathogens. Cytokines are glycoproteins involved in inflammation. Exercise training and physical activity are associated with a healthy lifestyle. In the current study, we aimed to determine the effects of high intensity interval training (HIIT) and L-Carnitine (LCAR) on the expression of genes involved in inflammation and inflammatory pathways in the liver and heart of rats. Method:Thirty-two male Wistar rats were randomly divided into the four groups (n = 8): 1. Untreated control group, 2. LCAR group (received 200 mg/kg LCAR daily), 3. HIIT group (performed high intensity interval training), 4. Exercise training + LCAR. Results:The results of our study showed that HIIT + LCAR significantly reduced the expression of IL-1β in the liver compared to the HIIT group (p = 0.038). The combination of HIIT and LCAR decreased IL-6 expression in the liver tissue compared to the control (p <0.001), LCAR (p <0.001), and HIIT (p = 0.002) groups. The HIIT + LCAR group decreased Cox 2 gene expression in the liver tissue compared to the untreated control group (p <0.001), and LCAR group (p = 0.007). The combination of HIIT and LCAR reduced IL-1β expression in the cardiac tissue compared to the untreated control (p <0.001), LCAR (p = 0.034), and HIIT (p = 0.041) groups. The combination of HIIT and LCAR increased IL-6 expression in the cardiac tissue compared to the other three groups (p <0.001). Conclusion:According to the obtained results, HIIT combined with LCAR administration is very useful in reducing the expression of pro-inflammatory genes in the heart and liver tissues.

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Cite This Study

Shahouzehi et al. (2021) studied Exercise-induced and baseline tissue inflammation in healthy male Wistar rats (n=32). High-intensity interval training (HIIT) combined with L-carnitine (LCAR) vs. Untreated control, L-carnitine alone (200 mg/kg/day), or HIIT alone was evaluated on Relative expression of pro-inflammatory genes (IL-1β, IL-6, TNF-α, and Cox-2) in liver and cardiac tissues (p=<0.001). High-intensity interval training plus L-carnitine significantly reduced hepatic IL-6 and Cox-2 and cardiac IL-1β expression compared with untreated controls in rats (all p<0.001).

synapsesocial.com/papers/6ae375204368cc01b5165e63 https://doi.org/10.22062/jkmu.2021.91564

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The Effect of High-intensity Interval Training and L-carnitine on the Expression of Some Pro-inflammatory Genes in the Liver and Cardiac Tissues of Rats

Key Points

PICO

Main Result

Limitations

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

The Effect of High-intensity Interval Training and L-carnitine on the Expression of Some Pro-inflammatory Genes in the Liver and Cardiac Tissues of Rats

Key Points

PICO

Main Result

Limitations

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

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