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Antibody staining and validation of intracellular and nuclear transcription factors for analysis CyTOF. The protocol outlines the preparation of fresh or cryopreserved cells, viability staining with Cell-ID Cisplatin, Fc receptor blocking, surface antibody staining, fixation and permeabilization, intracellular and nuclear antibody staining, and Iridium intercalation for sample preparation.
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Eduardo Maurício (2026) studied this question.
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