The Potency of Ipomoea pes-caprae Leaf Extract From North Lombok on Antiplatelet Activity in Balb/C Mice Strain

Antiplatelet agents are widely used for the secondary prevention of cerebrovascular disorders to reduce stroke risk, but their use is associated with adverse effects such as bleeding and drug resistance. Therefore, the development of novel antiplatelet agents, including those derived from natural products, is necessary. Ipomoea pes-caprae leaf extract has demonstrated stronger antiplatelet activity than the stem in vitro, with an inhibition concentration of 727.78 µg/mL. This study aimed to evaluate the antiplatelet aggregation effect of I. pes-caprae leaf extract by assessing bleeding and clotting time. An experimental study was conducted using Balb/C mice divided into a positive control group (aspirin 80 mg), a negative control group, and treatment groups receiving I. pes-caprae leaf extract at doses of 20, 40, 80, 100, and 120 mg/kg body weight. Bleeding and clotting times were measured before treatment (D0) and nine days after extract administration (D9). The results showed no statistically significant difference in bleeding time between the positive control and extract-treated groups at any dose. However, bleeding time was significantly increased in mice receiving extract doses of 40, 80, and 100 mg/kg body weight compared to the negative control (p < 0.05). A significant prolongation of clotting time was observed at a dose of 100 mg/kg body weight compared to the negative control (p < 0.05). In conclusion, I. pes-caprae leaf extract increases bleeding and clotting times, with the most pronounced effect at 100 mg/kg body weight, indicating its potential as a natural antiplatelet agent.