Screening of two bioactive compounds of Zingiber officinale Roscoe in morphine dependence

Introduction: Long-term of opioids can cause opioid dependence, which can lead to an opioid use disorder. The pattern and consequences of opioid use are characteristic of disorders classified as opioid use disorders.Objectives: To determine morphine dependence and other CNS effects of 6-gingerol and Zingerone on experimental models of rats.Materials and Methods: Albino Wistar male rats were screened for analgesic activity using Eddy's Hot Plate and Tail Flick Test, central depressant activity using the Open Field Test, hypnotic activity using the Thiopentone Sodium Hypnosis Potentiation Test. Straub's tail test, moderately and severely induced morphine dependence to rule out morphine-like activity.Results: Both phenolic compounds of ginger, 6-gingerol and zingerone, showed analgesic activity.6-Gingerol significantly extends the latency period and reduces the duration, and zingerone significantly prolongs the duration in the Thiopentone Induced Hypnosis Potentiation test. In the open field test, 6-gingerol showed a significant reduction in ambulation, and zingerone showed a significant increase in preening behavior. Straub tail test, showed a significant increase in latency and a decrease in duration. In moderate and severely induced morphine dependence, both zingerone and 6-gingerol showed a significant decrease in checked and counted signs as compared to the morphine group.Conclusion: 6-gingerol and zingerone have analgesic, anxiolytic, and anti-addictive properties, and 6-gingerol reduced the duration and zingerone increased the duration in the Thiopentone Sodium Hypnosis Potentiation Test.