In Vivo Skin Hydration Efficacy of Gel Body Lotion Formulated from Indramayu Mango (Mangifera indica L.) Peel Waste

Background: Indramayu mango (Mangifera indica L.) peel waste contains phenolic and flavonoid compounds that have the potential to inhibit the hyaluronidase enzyme from breaking down hyaluronic acid, thereby enhancing skin hydration. The study evaluated gel body lotion formulations containing Indramayu mango peel extract at varying concentrations (F0: 0%, F1: 0.15%, F2: 0.27%, and F3: 0.5%) on 28 female panelists aged 18–30 years. Objective: This study aims to evaluate the in vivo skin hydration efficacy of a gel body lotion formulated with Indramayu mango peel extract. Methods: The efficacy testing began with an irritation test (a 24-hour patch test), followed by measuring skin hydration levels on the back of the panelists hands over a 28-day period (days 0, 7, 14, 21, and 28) using a skin analyzer, as well as statistical data analysis using ANOVA and Duncan tests. Results: The irritation test indicated that all formulations were safe and non-irritating to the skin. Application of the gel body lotion significantly increased skin moisture. The highest average percentage increase in skin hydration was achieved by F3 (0.5%) at 107.98%, followed by F2 (0.27%) at 68.48%, F1 (0.15%) at 50.69%, and F0 (control) at 38.94%. Conclusion: Gel body lotion derived from Indramayu mango peel waste effectively increases skin hydration in vivo, with the 0.5% concentration providing the highest moisturizing efficacy.