Effects of different nutrient media on the growth and development of level III spawn of the golden oyster mushroom (Pleurotus citrinopileatus)

The golden oyster mushroom (Pleurotus citrinopileatus) is an edible mushroom with high nutritional value, rich in protein, vitamins, and minerals, and has potential applications in medicinal and functional foods.Research and refinement of the production process are crucial for improving the yield and quality of commercial mushrooms.This study aimed to evaluate the effects of different nutrient media on the growth and development of grade III spawn (P.citrinopileatus).Five nutrient media formulations were developed with different carbon, nitrogen, and mineral sources.The growth rate and morphological characteristics of the mycelium were monitored for 21 days under a temperature range of 24 -28°C.The results showed that formulation M4 (40.5% rice grain, 40.5% sawdust, 3% maize bran, 15% wheat bran, and 1% CaCO₃) resulted in faster mycelial growth, with dense, white, and uniformly distributed mycelium that firmly adhered to the substrate, reaching a mean mycelial length of 13.52cm.This research contributes to determining the optimal medium formula for the third stage of mushroom spawn production, laying the groundwork for large-scale propagation and improving the efficiency of cultivating golden oyster mushrooms.