The Analgesic and Anti-inflammatory Activities of Callerya speciosa Ethanol Extract in Mice

This study aimed to evaluate the analgesic and anti-inflammatory effects of a 70% ethanol extract from Callerya speciosa roots in Swiss mice. The extract was administered at doses of 400 and 1200 mg/kg. The analgesic activity was assessed using acetic acid–induced writhing, hot plate and formalin tests, while the anti-inflammatory activity was evaluated using carrageenan-induced acute peritonitis and cotton pellet–induced granuloma models. The extract exhibited significant peripheral analgesic activity in the acetic acid–induced writhing model, with the pain inhibition rates of 32.2% and 40.23% at 400 and 1200 mg/kg, respectively. In the formalin test, there was a significant reduction of nociceptive responses in the late phase (54.55% and 56.73%, respectively), but no significant effect in the hot plate model, indicating a lack of central analgesic activity. In the acute inflammation model, the extract reduced leukocyte infiltration (16.7% and 27.6%) and protein concentration (22% and 29.34%) in the peritoneal cavity exudate. In the chronic inflammation model, the extract at doses of 400 mg/kg and 1200 mg/kg significantly had granuloma formation inhibition with 19.40% and 21.49%, respectively. Overall, these findings suggest that Callerya speciosa extract exerts peripheral analgesic and anti-inflammatory effects, supporting its potential as a natural therapeutic agent for pain and inflammation management.