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Volatile components of oregano (Origanum vulgare L.) were extracted using headspace adsorptive microextraction and were analyzed by gas chromatography-mass spectrometry. Novel polyaniline-nylon-6 nanocomposite was used for headspace adsorptive microextraction due to its suitable characteristics. Multivariate curve resolution-alternating least square was applied for resolving the co-eluted gas chromatography-mass spectrometry peak clusters into pure chromatograms and mass spectra. Problems such as low signal-to-noise ratio of peaks and noisy data were handled with the aim of chemometrics. Chemicals of γ-terpinene, p-cymene, thymol, and carvacrol are the main components among 48 identified components of oregano fragrance. The volatile components of the fragrance and the essential oil of oregano were compared in term of chemical components and relative percentages points of view. The reproducibility in peak responses was investigated using three replicate experiments for the fragrance and the essential oil samples. The results proved that headspace adsorptive microextraction, gas chromatography-mass spectrometry, and multivariate curve resolution techniques could be useful to extract and analyze natural aromas and fragrances.
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Asadollahi-Baboli et al. (2014) studied this question.
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