Insights into the biotechnology of neglected Solanaceae species

This review article presents a comprehensive overview of recent advances in biotechnology, genetic transformation, and gene editing across eight wild and semi-domesticated species within the Solanaceae family. While these species are currently utilized in food, ethnomedicine, and various local applications, they have yet to achieve broad global cultivation. A detailed examination was conducted of each species’ potential for callus induction, shoot regeneration, genetic transformation, and gene editing. For comparative and statistical analyses, several parameters were assessed, including: the type and age of explants used for callus initiation, shoot regeneration, and microclonal propagation; optimal concentrations and combinations of growth regulators; and the overall efficiency of plant regeneration. The study identifies the most effective explant types and developmental stages and compiles a list of genes that have been successfully edited or stably integrated into plant genomes. Data presented in this review were statistically analysed and visualized using the Flourish online platform, with comparative findings presented in the form of a Sankey diagram.