Generation of four human induced pluripotent stem cell lines from dermal fibroblasts of healthy female donor

Four human induced pluripotent stem cell (hiPSC) lines were generated from commercially available dermal fibroblasts (Lonza, CC-2511) obtained from a healthy 39-year-old female donor using episomal plasmid-based reprogramming. Reprogramming was performed by either nucleofection (two lines) or lipofection (two lines), providing integration-free hiPSC lines. The undifferentiated state and pluripotency were confirmed by marker expression and trilineage differentiation assays, respectively, while genomic stability was confirmed by karyotypic analysis.