Optimization of dextran production in Leuconostoc mesenteroides BW2 using response surface methodology

This study aimed to verify the potential of Leuconostoc mesenteroides BW2 as an eco-friendly agricultural agent by optimizing its dextran production conditions and analyzing the molecular weight of the produced dextran.Using response surface methodology, the optimal cultivation parameters were determined to be 17.05% (w/v) sucrose, 24.48 o C, and an initial pH of 7.62, which yielded 1.333 g/100 mL of dextran, closely matching the predicted value.Additionally, gel permeation chromatography analysis revealed that the dextran produced at pH 7 exhibited a more uniform and higher molecular weight structure compared to that produced at pH 5 and 6.Finally, to facilitate practical agricultural application, the culture supernatant was formulated with chitosan.Particle size and zeta potential analyses confirmed that the dextran and other metabolites in the supernatant successfully bound with chitosan, resulting in charge neutralization and the formation of polydisperse microcomplexes.In conclusion, this study demonstrates the strong potential of L. mesenteroides BW2 for agricultural applications based on its dextran production.