A Multimodal Nano-Vehicle for the Transport of Biologically Active Amine Oxidase as a Therapeutic Enzyme

High polyamine (PA) concentration due to altered metabolism represents an Achilles’ heel in targeting PA-rich cancerous cells. This is due to the possibility of generating toxic by-products via the amine oxidase (AO)-catalyzed oxidation of PA. Nevertheless, AO intracellular delivery remains an open challenge. Recently, an extensive study on Caco-2 cells showed the applicability of a new magnetic and fluorescent nanoparticle (SAMN@TA) as a bovine serum amino oxidase (BSAO) carrier. Here, a SAMN@TA@BSAO multimodal nano-device was tested on two different human colorectal adenocarcinoma cell lines (LoVo WT and DX), confirming remarkable cell association along with a dose-dependent loss of cell viability, reaching 43% and 60% per single treatment, respectively. This contribution stimulates further investigation on SAMN@TA@BSAO use as a potential anticancer tool.