The complete mitochondrial genome and phylogenetic position of Acrocalymma sp. LF1 (Acrocalymmaceae) isolated from blueberry

Acrocalymma (Acrocalymmaceae, Dothideomycetes) mitogenomes remain largely uncharacterized, thereby restricting evolutionary analyses of this ecologically significant lineage. We assembled the Acrocalymma sp. LF1 mitogenome using Illumina short-read sequencing data, while performing phylogenetic analyses involving concatenated mitochondrial protein-coding sequences from 28 representative Dothideomycetes species. The assembled circular mitogenome consisted of 34,373 bp (AT content, 71.8%) and included 13 protein-coding genes, two ribosomal RNA genes, and 26 transfer RNA (tRNA) genes. Most protein-coding genes had ATG and TAA as start and stop codons, respectively, but cox1 had an incomplete stop codon (T—). Seven tRNAs had secondary structures that deviated from the canonical cloverleaf model. Additionally, two identical copies of the trnV-TAC tRNA gene were located at two distinct genomic loci. Phylogenetic analyses revealed that Acrocalymma sp. LF1 formed a fully supported sister relationship with Acrocalymma vagum in the family Acrocalymmaceae (bootstrap support = 100%). This study has enriched the mitogenomic dataset of Acrocalymmaceae and provides a fundamental genomic resource for subsequent systematic and evolutionary studies. Moreover, it confirms the utility of mitochondrial genomes for resolving phylogenetic relationships within this fungal lineage.