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The first complete mitochondrial genome (mitogenome) of Tetraodon pustulatus was sequenced, assembled, and annotated. The complete mitogenome is 16,471 bp in length and contains 37 mitochondrial genes, including 13 protein-coding genes (PCGs), two rRNA genes, and 22 tRNA genes, as well as one non-coding control region (D-loop). The overall nucleotide composition is A: 29.41%, T: 23.40%, C: 31.66%, G: 15.53%, with a GC content of 47.19%. Phylogenetic analysis revealed that T. pustulatus clustered within Tetraodon and was sister to T. lineatus, with 100% bootstrap support. This mitogenome provides a valuable molecular resource for species identification and phylogenetic studies of Tetraodontidae.
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Zhang et al. (2026) studied this question.
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