Hi-C Sequencing Facilitates an Improved Genome Assembly Resource of Colletotrichum fioriniae

Colletotrichum fioriniae is an ascomycete fungus that causes disease in a variety of crops, including strawberry, apple, and blueberry. Isolate 0001 was originally isolated from blueberry and was used to generate a long-read genome assembly. The assembly was then polished with short reads, and the contigs were scaffolded using Hi-C sequencing. The final annotated assembly contained 10 nuclear scaffolds and one mitochondrial scaffold that has a total size of 50.4 Mb and a scaffold N50 of 6.3 Mb. This genome is currently the most contiguous C. fioriniae genome available and will support future studies of population genetics, comparative genomics, and the development of molecular diagnostic tools.