Superior mitochondrial adaptations in human skeletal muscle after interval compared to continuous single‐leg cycling matched for total work

Key points A classic unresolved issue in human integrative physiology involves the role of exercise intensity, duration and volume in regulating skeletal muscle adaptations to training. We employed counterweighted single‐leg cycling as a unique within‐subject model to investigate the role of exercise intensity in promoting training‐induced increases in skeletal muscle mitochondrial content. Six sessions of high‐intensity interval training performed over 2 weeks elicited greater increases in citrate synthase maximal activity and mitochondrial respiration compared to moderate‐intensity continuous training matched for total work and session duration. These data suggest that exercise intensity, and/or the pattern of contraction, is an important determinant of exercise‐induced skeletal muscle remodelling in humans. Abstract We employed counterweighted single‐leg cycling as a unique model to investigate the role of exercise intensity in human skeletal muscle remodelling. Ten young active men performed unilateral graded‐exercise tests to measure single‐leg and peak power ( W peak ). Each leg was randomly assigned to complete six sessions of high‐intensity interval training (HIIT) 4 × (5 min at 65% W peak and 2.5 min at 20% W peak ) or moderate‐intensity continuous training (MICT) (30 min at 50% W peak ), which were performed 10 min apart on each day, in an alternating order. The work performed per session was matched for MICT (143 ± 8.4 kJ) and HIIT (144 ± 8.5 kJ, P > 0.05). Post‐training, citrate synthase (CS) maximal activity (10.2 ± 0.8 vs . 8.4 ± 0.9 mmol kg protein −1 min −1 ) and mass‐specific pmol O 2 •(s•mg wet weight) −1 oxidative phosphorylation capacities (complex I: 23.4 ± 3.2 vs . 17.1 ± 2.8; complexes I and II: 58.2 ± 7.5 vs . 42.2 ± 5.3) were greater in HIIT relative to MICT (interaction effects, P 0.05). In whole muscle, the protein content of COXIV (24%), NDUFA9 (11%) and mitofusin 2 (MFN2) (16%) increased similarly across groups (training effects, P 0.05). Single‐leg was also unaffected by training ( P > 0.05). In summary, single‐leg cycling performed in an interval compared to a continuous manner elicited superior mitochondrial adaptations in human skeletal muscle despite equal total work.