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September 10, 2025

Electroporation of Aiptasia zygotes v1

CRChristian CR RenickeJohns Hopkins University LLLorraine LingStanford University

Key Points

Electroporation allows transformation of hundreds to thousands of Aiptasia zygotes efficiently.
Genetic constructs include plasmid DNA, mRNA, and shRNA, facilitating various genetic modifications.
Larvae develop normally post-electroporation, enabling experimentation including symbiosis with algae.
This technique is accessible to anyone proficient in using a micropipette.

Abstract

This is a step-by-step guide to electroporation of Aiptasia (Exaiptasia diaphana) zygotes with different genetic constructs (plasmid DNA, mRNA, shRNA). It allows transformation of hundreds to thousands of zygotes in one session for anyone, who can hold and operate a micropipette. Larvae develop normally and can be used for experimentation, including establishment of symbiosis with compatible algal strains.

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Cite This Study

Renicke et al. (Sat,) studied this question.

synapsesocial.com/papers/68c189d99b7b07f3a061374c https://doi.org/https://doi.org/10.17504/protocols.io.rm7vzqdwrvx1/v1