Cell-free DNA from cerebrospinal fluid cytology specimens as a novel liquid biopsy approach for pediatric patients with primary central nervous system tumors

Key Points

• The central aim is to evaluate the feasibility of using cell-free DNA from cerebrospinal fluid for genomic studies in pediatric CNS tumors.
• Analyzed discarded supernatants from CSF cytology specimens.
• Performed liquid biopsy with whole-genome sequencing (LP-WGS) and targeted next-generation sequencing (NGS).
• Established that cell-free DNA from CSF can be used for liquid biopsy.
• Highlighted the potential limitations due to the quantity of CSF available in pediatric cases.

Abstract