Key points are not available for this paper at this time.
This protocol is used to extract genomic DNA from 0.22μm sterivex filters using Quiagens Blood and Tissue Kit. The protocol was developed as an equivalent replacement for the Phenol Chloroform extraction protocol. An additional PCR inhibitor removal step has been added to increase reproducibility for PCR-based downstream methods. This is especially important for coastal waters influenced by land runoff of glacial debris. This protocol is developed to work across all domains of life, from viruses to prokaryotes to eukaryotes, allowing for both amplicon sequencing and shotgun sequencing.
Andreas Novotny (Sat,) studied this question.