Development and validation of a sample-to-result real time multiplex RT-PCR test for human African trypanosomiasis and arboviral febrile illnesses in the Democratic Republic of the Congo

Key Points

• To develop and validate a rapid diagnostic test for human African trypanosomiasis and arboviral febrile illnesses.
• Created a real-time multiplex RT-PCR test.
• Tested the diagnostic accuracy in samples from affected individuals.
• Registered trial on ClinicalTrials.gov.
• The multiplex RT-PCR test demonstrated high sensitivity and specificity.
• Successful detection of both human African trypanosomiasis and arboviral infections was achieved.