Precision detection of rifampicin-resistant rpoB L378R mutation in Mycobacterium tuberculosis with CRISPR-Cas12a

Key Points

• This research aims to improve the detection of rifampicin-resistant mutations in Mycobacterium tuberculosis using a specific assay.
• Developed a novel RPA-CRISPR-Cas12a assay
• Targeted the rpoB L378R mutation
• Utilized molecular techniques for detection
• Successfully detected the rpoB L378R mutation
• Demonstrated high sensitivity and specificity for rifampicin resistance
• Indicated improved detection capabilities compared to conventional methods