What question did this study set out to answer?

This research aims to compare the antimicrobial efficacy of various substances on dental biofilm.

February 19, 2026BMC Oral Health2 citationsOpen Access

Antimicrobial effects of Propolis, calcium Hydroxide, triple antibiotic paste, and modified triple antibiotic paste on tubular dentin inoculated with a dual-species biofilm: an ex vivo study

ZAZohreh AhangariShahid Beheshti University NZNazanin ZargarShahid Beheshti University MPMaryam Pourhajibagher

Key Points

This research aims to compare the antimicrobial efficacy of various substances on dental biofilm.
Ex vivo analysis of antimicrobial effects on tubular dentin inoculated with dual-species biofilm.
Comparison of Propolis, calcium hydroxide, triple antibiotic paste, and modified triple antibiotic paste.
Evaluation of effectiveness against E. faecalis and A. naeslundii.
Triple antibiotic paste and modified triple antibiotic paste showed high antimicrobial efficacy.
Calcium hydroxide demonstrated acceptable antimicrobial efficacy.
Propolis exhibited lower efficacy in eliminating the biofilm.

Abstract

TAP and mTAP demonstrated high efficacy, and CH showed acceptable efficacy for elimination of E. faecalis and A. naeslundii dual-species biofilm. Propolis showed lower efficacy, highlighting the need for further modifications to enhance its penetration depth.

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