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The research aims to optimize protocols for collecting and analyzing microRNA from stool samples of infants and toddlers.

February 22, 2026RNA Biology0 citationsOpen Access

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Optimizing protocols for MicroRNA profiling of infant and Toddler Stool

DADavid A. ArmstrongDartmouth Hospital SSShannon M. SoucyDartmouth College MMMeghan E. MuseDartmouth College

Key Points

The research aims to optimize protocols for collecting and analyzing microRNA from stool samples of infants and toddlers.
Collected stool samples from infants and toddlers under 2 years using RNAlater and Zymo DNA/RNA Shield Tubes.
Employed RNA extraction via the Zymo BIOMICs extraction kit.
Compared miRNA profiles from different collection methods.
High-quality RNA was obtained regardless of stool collection method.
Human miRNA profiles were similar across both RNAlater and Zymo DNA/RNA Shield collections.

Abstract

Collecting stool from infants and toddlers < 2 years of age in either RNAlater or Zymo DNA/RNA Shield Faecal Collection Tubes, when paired with RNA extraction using the Zymo BIOMICs extraction kit, yielded high-quality RNA with similar human miRNA profiles.

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Optimizing protocols for MicroRNA profiling of infant and Toddler Stool

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