What does this research mean for the field?

A colorimetric LAMP assay using cresol red, combined with an intelligent smartphone application, enables automated, high-throughput visual detection of Mycoplasma gallisepticum with 98% concordance to standard qPCR. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To develop a rapid and reliable method for detecting Mycoplasma gallisepticum using colorimetric LAMP and smart technology.

March 29, 2026Analytical Methods0 citations

High-throughput colorimetric LAMP detection of Mycoplasma gallisepticum with intelligent algorithm-assisted analysis

WJWuyang JingSouth China Agricultural University QCQianyi CaiSouth China Agricultural University YLYucen LiangSouth China Agricultural University

Key Points

To develop a rapid and reliable method for detecting Mycoplasma gallisepticum using colorimetric LAMP and smart technology.
Utilized a colorimetric LAMP assay with cresol red for visual detection.
Developed a smartphone application (Lab Color Analyzer) for automated sample analysis.
Tested the assay performance against qPCR for accuracy.
Achieved 98% concordance with qPCR for detecting Mycoplasma gallisepticum.
Enabled analysis of up to 64 samples simultaneously.
Provided rapid visual detection of the bacteria.

Abstract

A colorimetric LAMP assay using cresol red enables visual detection of Mycoplasma gallisepticum , while an intelligent smartphone application (Lab Color Analyzer) automates analysis of up to 64 samples, achieving 98% concordance with qPCR.

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