What question did this study set out to answer?

The research aims to clarify how N-acetylglucosamine-6-O-sulfotransferases determine the specific ways sulfated O-glycans are formed.

April 15, 2026Organic Chemistry Frontiers1 citationsOpen Access

Defining Substrate Specificities of N-Acetylglucosamine-6-O-Sulfotransferases for Enzymatic Modular Assembly of Sulfated O-Glycans

SFShuquan Fan JHJinghua HanGeorgia State University TSTangliang ShenGeorgia State University

Key Points

The research aims to clarify how N-acetylglucosamine-6-O-sulfotransferases determine the specific ways sulfated O-glycans are formed.
Analyzed substrate specificities of N-acetylglucosamine-6-O-sulfotransferases.
Examined enzymatic reactions to assess regioselectivity in sulfation.
Utilized biochemical assays to test the efficacy of sulfotransferases.
Identified specific patterns of substrate recognition by sulfotransferases.
Demonstrated improved regioselective sulfation for targeted O-glycan synthesis.
Highlighted the potential for enhanced glycan engineering through defined enzyme specificities.

Abstract

Sulfated O-glycans are widely distributed and play key roles in a wide range of physiological and disease processes, yet their synthesis remains challenging due to difficulties in regioselective sulfation. Here,...

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