Rapid and portable molecular test for Mycobacterium sp based on double-tagged amplification and electrochemical readout

Key Points

• The central aim is to develop a rapid and portable test for detecting Mycobacterium tuberculosis.
• Utilized double-tagged amplification for enhancing sensitivity and specificity.
• Employed an electrochemical readout for quick analysis.
• Conducted tests in various conditions to assess portability and rapidity.
• Successfully detected Mycobacterium tuberculosis with a sensitivity of 95% and specificity of 90%.
• Test results were available in under 30 minutes, significantly faster than standard methods.
• Showed consistent performance across different sample types.