Uncontrolled hemorrhage and subsequent infection severely impair wound healing. This study developed a multifunctional Polyamidoamine Dendrimer/Sodium Alginate/γ-polyglutamic Acid/Amoxicillin (G3/SA/γ-PGA/AMX) hydrogel via 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide activated amidation. The hydrogel featured a porous structure, balanced mechanical properties, excellent self-healing ability, and injectability. In SD rat liver bleeding and tail amputation models, it significantly outperformed commercial gelatin sponge, reducing blood loss to 0.09 ± 0.02 g and 0.04 ± 0.01 g respectively, with hemostatic time shortened to 0.69 ± 0.17 min and 0.62 ± 0.10 min. Sustained amoxicillin release conferred over 96% bactericidal rate against Escherichia coli and Staphylococcus aureus. It exhibited negligible hemolysis and high L929 cell viability (>90%), ensuring good biocompatibility. In C57 mice scald models, the hydrogel promoted rapid wound healing with a rate exceeding 98.91 ± 1.04% after 20 days, accelerating epidermal regeneration and dense collagen deposition. These results highlight the hydrogel's superior hemostatic and wound healing capabilities, making it a promising candidate for clinical wound management.
Jia et al. (Tue,) studied this question.