Transcriptome-constrained genome-scale metabolic modeling reveals central carbon and amino acid metabolic reprogramming underlying colistin-sulbactam synergy in Acinetobacter baumannii

Key Points

• This research aims to explore how combination therapy influences metabolic pathways in Acinetobacter baumannii.
• Utilized transcriptome-constrained genome-scale metabolic modeling to analyze metabolic shifts.
• Examined changes in central carbon and amino acid metabolism in the presence of colistin-sulbactam.
• Combination therapy leads to a shift from central carbon metabolism to amino acid and ion metabolism.
• Identified key enzymes such as isocitrate lyase and malate synthase as potential targets to improve antimicrobial synergy.