This research aims to characterize yeast ribosomal populations and investigate their functional changes during polysome collapse.
Utilized offline two-stage size exclusion chromatography (SEC) combined with LC-MS/MS for analysis.
Focused on detecting changes in ribosome composition during functional perturbations.
Employs polysome profiling to assess the interactions and compositions of ribosomal populations.
Demonstrated rapid polysome collapse in yeast under knockout conditions.
Detected significant alterations in polysome profiling ratios linked to ribosomal composition changes.
Abstract
knockout, we show that 2D-SEC MS/MS detects rapid polysome collapse and changes to the polysome profiling ratios, illustrating its capacity to link ribosome composition to functional perturbations.