Evaluation of vardenafil's metabolic stability in human liver microsomes through ultra-fast UPLC-MS/MS for quantitative analysis, including assessments of greenness, ADME properties, DEREK alerts, and metabolic lability

Key Points

• This research aims to assess the metabolic stability of vardenafil using an ultra-fast UPLC-MS/MS technique.
• Utilized UPLC-MS/MS for the first time to quantify vardenafil in human liver microsomes (HLMs).
• Evaluated greenness, ADME properties, and DEREK alerts related to vardenafil derivatives.
• Demonstrated that small changes to the 4-ethylpiperazine ring could enhance metabolic stability of new derivatives.