Inoculation with human adenovirus-37 significantly increased visceral and total body fat (P<0.001) and reduced serum triglycerides (P<0.0001) in chickens compared with controls.
Does inoculation with human adenoviruses (Ad-2, Ad-31, Ad-37) increase adiposity and alter serum lipids in chickens and 3T3-L1 cells?
Ad-37 is identified as another human adenovirus that increases adiposity and alters serum lipids in an animal model, expanding the link between viral infection and obesity.
p-value: p=<0.001
Human adenovirus-36 (Ad-36) increases adiposity in chickens, mice, and nonhuman primates and is associated with human obesity. Ad-36 paradoxically reduces serum cholesterol and triglycerides in animal models. Ad-36 increases adipocyte differentiation and triglyceride accumulation in 3T3-L1 cells in vitro. This study evaluated whether three other human adenoviruses increase adiposity in chickens and in 3T3-L1 cells in vitro. We inoculated chickens with human Ad-2, Ad-31, Ad-37, or media at age 3 wk. Food intake and weights were recorded for 3.5 wk, and then chickens were killed and visceral fat, body composition, serum lipids, and viral antibody status were determined. Visceral fat and total body fat were significantly elevated (P < 0.001) in the Ad-37 group compared with all other groups. Final body weights were higher in chickens inoculated with Ad-37 compared with Ad-2, but not significantly higher than in control or Ad-31 groups. Food intake did not differ among groups. Serum cholesterol was elevated in Ad-37 chickens compared with control (P < 0.01) but was not affected by other viruses. Triglycerides were reduced in Ad-37 chickens (P < 0.0001) but were not affected by other viruses. In 3T3-L1 cells in vitro, Ad-31, Ad-36, and Ad-37, but not Ad-2, increased adipocyte differentiation and triglycerides accumulation. In summary, Ad-37 is another human adenovirus that increases adiposity and reduces serum triglycerides in an animal model. However, the response of serum cholesterol is opposite that of Ad-36. Evaluation of other human adenoviruses to determine their effects on adiposity and serum lipids is warranted, but in vitro assays may not be definitive for this purpose.
Whigham et al. (Fri,) conducted a other in Adiposity. Human adenoviruses (Ad-2, Ad-31, Ad-37) vs. Media (control) was evaluated on Visceral fat and total body fat (p=<0.001). Inoculation with human adenovirus-37 significantly increased visceral and total body fat (P<0.001) and reduced serum triglycerides (P<0.0001) in chickens compared with controls.