Electrical stimulation of a two-dimensional sheet of myocardial cells in a perfusing bath results in depolarization under the electrode and hyperpolarization on each side along the fiber axis.
A mathematical model of cardiac cell monolayers shows that a perfusing bath alters the quantitative but not qualitative spatial distribution of transmembrane potential during electrical stimulation, supporting the use of superfused monolayers to study break excitation and reentry.
BACKGROUND: The goal of our study is to examine the effect of stimulating a two-dimensional sheet of myocardial cells. We assume that the stimulating electrode is located in a bath perfusing the tissue. METHODS: An equation governing the transmembrane potential, based on the continuity equation and Ohm's law, is solved numerically using a finite difference technique. RESULTS: The sheet is depolarized under the stimulating electrode and is hyperpolarized on each side of the electrode along the fiber axis. CONCLUSIONS: The results are similar to those obtained previously by Sepulveda et al. (Biophys J, 55: 987-999, 1989) for stimulation of a two-dimensional sheet of tissue with no perfusing bath present.
Srinivasan et al. (Tue,) conducted a other in Cardiac electrical stimulation. Electrical stimulation via point electrode in a perfusing bath was evaluated on Transmembrane potential distribution. Electrical stimulation of a two-dimensional sheet of myocardial cells in a perfusing bath results in depolarization under the electrode and hyperpolarization on each side along the fiber axis.