Key result
Electrical stimulation of a two-dimensional sheet of myocardial cells in a perfusing bath results in depolarization under the electrode and hyperpolarization on each side along the fiber axis.
A mathematical model of cardiac cell monolayers shows that a perfusing bath alters the quantitative but not qualitative spatial distribution of transmembrane potential during electrical stimulation, supporting the use of superfused monolayers to study break excitation and reentry.
Does not support altering clinical stimulation protocols; extends monolayer models to perfusing baths for reentry studies.
BACKGROUND: The goal of our study is to examine the effect of stimulating a two-dimensional sheet of myocardial cells. We assume that the stimulating electrode is located in a bath perfusing the tissue. METHODS: An equation governing the transmembrane potential, based on the continuity equation and Ohm's law, is solved numerically using a finite difference technique. RESULTS: The sheet is depolarized under the stimulating electrode and is hyperpolarized on each side of the electrode along the fiber axis. CONCLUSIONS: The results are similar to those obtained previously by Sepulveda et al. (Biophys J, 55: 987-999, 1989) for stimulation of a two-dimensional sheet of tissue with no perfusing bath present.
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Srinivasan et al. (2004) studied Cardiac electrical stimulation. Electrical stimulation via point electrode in a perfusing bath was evaluated on Transmembrane potential distribution. Electrical stimulation of a two-dimensional sheet of myocardial cells in a perfusing bath results in depolarization under the electrode and hyperpolarization on each side along the fiber axis.
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