Complex III enzymatic activity was lower in patients with idiopathic and ischemic dilated cardiomyopathy compared with normal controls, suggesting it is a secondary phenomenon.
Case-Control (n=40)
Are mitochondrial respiratory chain enzyme activities altered as a primary or secondary phenomenon in patients with idiopathic dilated cardiomyopathy?
The decrease in respiratory chain complex III activity in idiopathic dilated cardiomyopathy is likely a secondary phenomenon rather than a primary mitochondrial disease.
OBJECTIVE: To study the mitochondrial respiratory chain enzyme activities in patients with idiopathic dilated cardiomyopathy (IDC). METHODS: Mitochondrial respiratory chain enzyme activities were assessed spectrophotometrically in left ventricular tissue of 17 patients with IDC undergoing cardiac transplantation, as well as in two groups of controls: a group of six patients suffering from ischemic dilated cardiomyopathy (IC) also undergoing cardiac transplantation, and a group of 17 organ donors considered normal from a cardiac point of view. Cytochrome b gene from three IDC patients whose complex III activity was particularly low and from three controls was also sequenced. RESULTS: We found that complex III enzymatic activity was lower not only in IDC but also in IC patients when compared with normal controls. When analysing cytochrome b gene we only found neutral polymorphisms previously described. CONCLUSIONS: In view of such results, we believe that the decrease of respiratory chain complex III activity found in some cases of IDC is a secondary phenomenon, and not due to a primary mitochondrial disease.
Diana Jarreta (Wed,) conducted a case-control in Idiopathic dilated cardiomyopathy (n=40). Idiopathic dilated cardiomyopathy vs. Ischemic dilated cardiomyopathy and normal organ donors was evaluated on Mitochondrial respiratory chain enzyme activities (complex III). Complex III enzymatic activity was lower in patients with idiopathic and ischemic dilated cardiomyopathy compared with normal controls, suggesting it is a secondary phenomenon.