What type of study is this?

This is a In Vitro Study study.

August 18, 2025Open Access

Figure S3 from PSA Secretion from Single Circulating Tumor Cells of Metastatic Castration-Naïve Prostate Cancer Patients

Key Points

PSA secretion was effectively visualized from individual circulating tumor cells (CTCs), showcasing tumor-specific activity.
Analysis included printing PSA proteins onto PVDF membranes to facilitate matching with CTCs for deeper insights.
The process employed nanowell arrays, enhancing the ability to monitor single-cell behavior and characteristics effectively.
Findings support the potential for isolating viable CTCs for further investigations into prostate cancer progression.

Abstract

Visualization of the nanowells and membrane using the VyCAP SPOT software (A) Imprint of IgG antibody printed onto a PVDF membrane using a nanowell array, is used to align and match the secreted proteins to the corresponding cell inside a well. (B) The PSA proteins secreted by the individual CTC is printed onto PVDF membrane. (C) A brightfield image of the nanowell arrays filled with CTC. (D)Viable CTC inside individual wells of the chip. (E) PSMA-PE expression of the CTC inside the nanowells. (F) CD45-APC expression of the CTC inside the chip. Images in the lower left corner illustrate a small part of the membrane showing the IgG spots (A) and PSA spots (B) with their corresponding well-id analyzed in the VyCAP SPOT software. The corresponding CTC secreting PSA can now be located inside the nanowell array (C-F) to determine cell phenotype and isolated for downstream analysis.

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Cite This Study

Dathathri et al. (Mon,) studied this question.

synapsesocial.com/papers/68af4328ad7bf08b1ead2228 https://doi.org/https://doi.org/10.1158/2767-9764.29929861

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