What type of study is this?

This is a Experimental Study study.

October 9, 2025Open Access

Oxford Nanopore Technologies (ONT) direct RNA sequencing v1

AAAnita AdamiLund University RGRaquel GarzaLund University

Key Points

This protocol covers the entire workflow of ONT direct RNA sequencing, ensuring reliable results.
Key steps include RNA extraction, mRNA poly(A) selection, and library preparation for sequencing.
Direct RNA sequencing offers advantages in capturing full-length transcripts and complex RNA structures.
Efficient data analysis methods are crucial for interpreting results from ONT direct RNA sequencing.

Abstract

Step-by-step protocol describing the workflow for ONT direct RNA sequencing including: - RNA extraction - mRNA poly(A) selection - library preparation for ONT direct RNA sequencing - ONT direct RNA data analysis

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Cite This Study

Adami et al. (Fri,) studied this question.

synapsesocial.com/papers/68e70db290569dd607ee61f3 https://doi.org/https://doi.org/10.17504/protocols.io.36wgqpeo3vk5/v1

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