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PicoQuant`s new confocal microscope Luminosa combines state-of-the-art hardware with cutting edge software to deliver high quality data while simplifying daily operation. Luminosa's rapidFLIM hardware can record several frames per second, which the software handles with a novel dynamic binning format. In combination with GPU-accelerated algorithms, this enables high-speed automated analysis of FLIM images. We demonstrate the combination of PicoQuant's latest multi-channel TCSPC device and a cooled high-performance 23-pixel SPAD-array developed jointly with Pi Imaging Technologies for time-resolved confocal image scanning microscopy (FLIM-ISM). We also discuss how advanced data processing can be applied to FLIM-ISM for additional performance gains. For certain applications widefield imaging offers unique advantages. Here we show how the combination of a powerful PicoQuant laser and the novel SPAD512S camera from Pi Imaging Technology facilitates time-resolved widefield imaging. We benchmark the camera's performance, demonstrating video rate acquisition speeds with minimal photobleaching.
Koberling et al. (Wed,) studied this question.
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