August 5, 2025PlantsOpen Access

Accumulating Heterozygous Deleterious Mutations in Conserved Soybean Germplasm over Successive Regenerations

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Total mutation burden in soybean germplasm increases significantly with years of conservation and number of regenerations.
RNA-Seq analysis revealed 588 deleterious variants distributed across 20 soybean chromosomes in 190 accessions.
Heterozygous mutations were primarily responsible for the increase in total mutation burden, while homozygous mutations showed no significant correlation.
Understanding mutation accumulation aids in the management and conservation of diverse soybean germplasm collections.

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Seed-based RNA-Seq identifies increasing heterozygous mutations in soybean accessions, suggesting conservation impacts.

Fu et al. (2025) studied this question.

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