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This research aims to measure the change in deleterious mutation burden in barley germplasm over one regeneration cycle.

Measuring the change in deleterious mutation burden of barley germplasm conserved over one regeneration cycle

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Randomized trial measures deleterious mutation burden in barley germplasm, suggesting mutation accumulation during conservation.

This research aims to measure the change in deleterious mutation burden in barley germplasm over one regeneration cycle.
Seed-based RNA-Seq analysis was conducted on paired samples of 90 barley accessions (G0 and G1).
Two SNP calling methods were used to identify deleterious variants distributed across seven chromosomes.
Changes in total mutation burden per deleterious locus were estimated based on Bcftools SNP calls.
1,642 deleterious variants were identified, predominantly occurring in heterozygotes.
The estimated accumulation was 28 to 37 deleterious variants over the G0–G1 period.
More deleterious variants were found in G1 samples compared to G0, with unique variants in each sample type.

Yong‐Bi Fu (2026) studied this question.

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