Periodic structured illumination improves voltage imaging in awake mice, indicating better signal quality than traditional methods.
We utilize periodic structured illumination with pseudo-HiLo (pHiLo) image reconstruction for in vivo voltage imaging. We demonstrate reduced signal from out-of-focus cells, that contaminates voltage activity for in-focus cells of interest, with pHiLo compared to traditional widefield recordings taken with uniform illumination and pseudo-widefield (pWF) reconstructions. We discuss tradeoffs between signal-to-background ratio, signal-to-noise ratio and temporal resolution for pHiLo in the context of high-speed voltage imaging in awake mice.
No takes yet. Share an insight, caveat, or question.
Speed et al. (2025) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: