August 3, 2025Open Access

Vacuolar Phosphatidylinositol 3,4,5-trisphosphate controls fusion through binding Vam7, and membrane microdomain assembly

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Discussion

In vitro assays demonstrate that PI(3,4,5)P3 affects fusion in vacuoles, suggesting its role in microdomain assembly and SNARE function.

Vacuolar PI(3,4,5)P3 is produced from PI(4,5)P2 via class III PI 3-kinase, influencing vesicle fusion.
Blocking PI(3,4,5)P3 with C8 or PTEN prevents the fusion of vesicles, highlighting its significance in the process.
Fluorescence microscopy reveals that PI(3,4,5)P3 accumulates in membrane microdomains, essential for vesicle traffic.
The interaction between Vam7 and PI(3,4,5)P3 is crucial for assembling SNARE complexes necessary for fusion.

Zhang et al. (2025) studied this question.

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