August 3, 2025Open Access

A Sequence Motif Enables Widespread Use of Non-Canonical Redox Cofactors in Natural Enzymes

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Authors

SSSamer SalehUniversity of California, Irvine NHNing-Hsiang HsuUniversity of California, Irvine ELEmma LuuUniversity of California, Davis

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Implication

Screening identifies RH/QxxR motif that improves redox cofactor activity in aldehyde dehydrogenase enzymes, highlighting potential for biomanufacturing.

Key Points

The RH/QxxR sequence motif enables enhanced redox cofactor activity in aldehyde dehydrogenases.
Bos taurus ALDH3a1 shows k cat values for NMN + that exceed those of traditional NAD + by up to 10 to 10 5 -fold.
Structural analyses indicate the motif helps position the cofactor effectively within the active site of enzymes.
This work provides a strategy to engineer enzymes for improved biomanufacturing using non-canonical redox cofactors.

Cite This Study

Saleh et al. (2025) studied this question.

synapsesocial.com/papers/689a0c7be6551bb0af8d05ed https://doi.org/10.1101/2025.08.01.668186

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Implication

Screening identifies RH/QxxR motif that improves redox cofactor activity in aldehyde dehydrogenase enzymes, highlighting potential for biomanufacturing.

A Sequence Motif Enables Widespread Use of Non-Canonical Redox Cofactors in Natural Enzymes

Discussion

Member takes

Implication

Key Points

Cite This Study

A Sequence Motif Enables Widespread Use of Non-Canonical Redox Cofactors in Natural Enzymes

Discussion

Member takes

Implication

Key Points

Cite This Study

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