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August 7, 2025American Journal of Tropical Medicine and Hygiene

Polymerase Chain Reaction-Based Diagnosis of Leishmaniasis Targeting Kinetoplast Minicircle DNA in Infants with Hemophagocytic Lymphohistiocytosis

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Authors

HZHeng ZhaoSSShulan ShiYZYing Zhang

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Overview

This investigation demonstrates effective diagnosis of VL-HLH in infants using PCR targeting kDNA, indicating its potential for clinical application.

Key Points

  • Polymerase chain reaction successfully detected Leishmania in infants with hemophagocytic lymphohistiocytosis.
  • kDNA PCR yielded specific amplification and quantification results, with copy numbers reaching up to 77,168/µL.
  • Real-time quantitative PCR accurately determined parasite load and facilitated species identification in patient samples.
  • The study supports kDNA PCR as a reliable diagnostic tool for visceral leishmaniasis.

Cite This Study

Zhao et al. (2025) studied this question.

synapsesocial.com/papers/689dfe90d61984b91e13bc77https://doi.org/10.4269/ajtmh.24-0792
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