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August 11, 2025Anti-Infective Agents

Spectrofluorimetric and Spectrophotometric Methods for the Determination of Ciprofloxacin HCl and Their Comparison with the Compendial High- Performance Liquid Chromatography (HPLC) Method

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Authors

MKMarium Fatima KhanBaqai Medical UniversityMSMuhammad Ali SherazBaqai Medical UniversitySASofia AhmedBaqai Medical University

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Implication

Spectrophotometric and spectrofluorimetric methods accurately quantify ciprofloxacin in pharmaceutical formulations, indicating robustness compared to HPLC.

Key Points

  • Both spectrophotometric methods showed excellent precision, with recovery values ranging from 98.60% to 100.32%.
  • Validation was conducted following ICH guidelines, demonstrating low limits of detection at 0.64 and 0.66 µM for UV and fluorescence methods respectively.
  • Comparative analysis demonstrated that the results from the UV and fluorescence methods align closely with the compendial HPLC results.
  • UV methods are accessible for basic labs, while fluorescence provides enhanced sensitivity for quantifying low concentrations.

Cite This Study

Khan et al. (2025) studied this question.

synapsesocial.com/papers/68a360d60a429f7973328ed8https://doi.org/10.2174/0122113525386386250704103943
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