Self-calibrated surface plasmon resonance imaging detection method using wavelength interrogation

This paper reports a wavelength interrogation-based self-calibrated surface plasmon resonance imaging (SPRi) detection method through simulation. The SPRi detection device utilizes the wavelength scanning technique in combination with a voltage-adjusted transmittance device to solve the problem of polarization filter transmission differences at different wavelengths. By collecting data at different wavelengths and applying a differential operation algorithm to calculate both bulk and conjugate refractive index changes without an extra calibration step, the reported method simplifies the detection process, reduces the calculation time, and lowers the device cost. Thus, the paper provides a more efficient and economical solution in the field of biochemical substance detection.