Study Factors Affecting the Content of Essential oil of Vernonia amygdalina and Alphaglucosidase Inhibitory Activity

From the fresh leaves of the Vernonia amygdalina, an essential oil was obtained by hydrodistillation. The essential oil was obtained by steam distillation using a plant material-to-water ratio of 1:8 (w/v), conducted over 2.5 hours at a temperature range of 60–80 °C. The resulting essential oil was pale yellow in color, with a yield of 0.75%.In addition, we also tested the anti-α-glucosidase activity of Vernonia amygdalina essential oil. The results showed that the essential oil also exhibited strong activities with an IC50 value of 3.31 ± 0.04 mg/ml. These findings underscore the potential of Vernonia amygdalina essential oil as a natural source for pharmaceutical applications