Figure 5 from Collagen XVII Promotes Pancreatic Ductal Adenocarcinoma Tumor Growth through Regulation of PIK3R5

Reduced tumor forming potential of COL17A1-deficient PDAC cells. A, Schematic of orthotopic implantations of PDAC cells into the pancreata of NSG mice and photographs of orthotopic tumors formed by MGH1319 and MGH1275 cells expressing NTC and COL17A1-specific shRNAs. Scale bars, 1 cm. B and C, MGH1319 expressing NTC and COL17A1-specific shRNAs were implanted into the pancreas of mice. Tumor volumes were calculated by caliper measurement (B), and mass was determined (C). Data are presented as mean (±SEM) from five mice per group. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (one-way ANOVA with Dunnett’s post hoc test). D, Representative images of collagen XVII IHC (top) and hematoxylin and eosin (H&E) staining (bottom). Scale bars, 50 µm. E and F, MGH1275 cells expressing NTC and COL17A1-specific shRNAs were implanted into the pancreas of mice. Tumor volumes were calculated by caliper measurement (E) and mass determined (F). Data are presented as mean (±SEM) from five mice per group. ****, P < 0.0001 (one-way ANOVA with Dunnett’s post hoc test). G, Representative images of collagen XVII IHC (top) and H&E staining (bottom). Scale bars, 50 µm. H, IHC staining of pan-cytokeratin (red) and Ki-67 (brown). I and J, Percentage of Ki-67–positive cancer cells in tumors formed by MGH1319 cells (I) and MGH1275 cells (J) expressing NTC and COL17A1-specific shRNAs. The average (±SEM) from four tumors is shown. ***, P < 0.001; ****, P < 0.0001 (one-way ANOVA with Dunnett’s post hoc test).