Figure 2 from SREBP-Dependent Regulation of Lipid Homeostasis Is Required for Progression and Growth of Pancreatic Ductal Adenocarcinoma

SCAP is required for human PDAC tumor growth in mouse subcutaneous xenograft models. A, Nude mice were subcutaneously injected with 5 × 10⁵ Pa03c cells in both flanks (two tumors per mouse). Once visible, tumors were measured, and volume calculated. Each group contained six mice. Error bar denotes SD. Statistical significance was determined using the Student t test. P values are indicated: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant. B, Individual tumor volumes at day 22, n = 12 tumors per group. Statistical significance was determined using the Student t test. C, Representative hematoxylin and eosin–stained sections of formalin-fixed tumor tissue from the mice in A showing a low magnification (4×) image (top row) and a higher magnification (20×) image (bottom row) of tumor sections. D, Nude mice were subcutaneously injected with 1 × 10⁶ Pa16c cells in both flanks (two tumors per mouse) as in A–C. Each group contained six mice. Error bar denotes SD. Statistical significance was determined using the Student t test. P values are indicated: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant. E, Individual tumor volumes at day 61, n = 12 tumors per group. Statistical significance was determined using the Student t test. F, Representative hematoxylin and eosin–stained sections of formalin-fixed tumor tissue from the mice in D showing a low magnification (2–4×) image (top row) and a higher magnification (20–40×) image (bottom row) of tumor sections. WT, wild-type.