Protocol outlines fractionating complex mixtures using wide-range-polarity method on Isolera-Biotage system, suggesting improved separation.
This protocol describes the procedure for fractionating complex mixtures using the Wide-Range-Polarity (WRP) method on the Isolera-Biotage system. This method employs a controlled elution gradient to separate compounds with a wide range of polarity, such as secondary metabolites from natural extracts. The method is applicable to silica gel or C18 stationary phase columns, depending on the sample characteristics.
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Borges et al. (2025) studied this question.
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