Exogenous Dopamine Alleviates Combined High Temperature and Drought Stress in Loquat [Eriobotrya japonica (Thunb.) Lindl.] Seedlings: Improvements in Photosynthetic Efficiency, Oxidative Damage and Osmotic Regulation

In recent years, high temperature and drought have severely impacted the growth and development of loquat [Eriobotrya japonica (Thunb.) Lindl.] plants. Although dopamine can improve the stress resistance of plants, its role in combined stress requires further exploration. This study investigated the alleviative effect and mechanism of exogenous dopamine on loquat seedlings subjected to the combined stress of high temperature and drought. The combined stress significantly reduced root viability, photosynthetic pigment content, and net photosynthetic rate (Pn) while markedly increasing reactive oxygen species (ROS) levels, thiobarbituric acid-reactive substances (TBARS) content, and electrolyte leakage (EL). The seedlings exhibited pronounced wilting symptoms, along with markedly reduced root surface area and volume. Dopamine treatment significantly alleviated combined stress-induced damage. This mitigation was manifested through substantially enhanced root viability, photosynthetic pigment content, Pn, antioxidant enzyme activities, and osmotic adjustment substances concomitantly with marked reductions in ROS, TBARS content, and EL. Dopamine significantly reduced seedling wilting severity and improved root morphological parameters. This study demonstrates that dopamine enhances loquat seedlings’ tolerance to combined stress through coordinated mechanisms: maintaining photosynthetic pigments and improving stomatal conductance to sustain photosynthetic efficiency, enhancing antioxidant enzyme activity and ROS scavenging capacity to mitigate oxidative damage, and promoting osmotic solute accumulation for osmotic potential regulation.